| Team | State | Region |
| 6-4-3 DP Academy | Georgia | Deep South |
| ABD Boxers | California | West Coast |
| ABD Orange County | California | West Coast |
| All American Prospects | Florida | Sunshine |
| Allegheny Tigers | Pennsylvania | Ohio Valley |
| Arlington A's | Texas | Sun Belt |
| Austin Slam | Texas | Sun Belt |
| Austin Wings | Texas | Sun Belt |
| Bandits Baseball | Pennsylvania | Ohio Valley |
| Baseball U | New Jersey | New England |
| Burkburnett Black Sox | Texas | Sun Belt |
| Cape Coral Cannons | Florida | Sunshine |
| Cincy Flames | Ohio | Ohio Valley |
| Columbus Cobras | Ohio | Ohio Valley |
| Cyclones Baseball | Florida | Sunshine |
| DBAT Mustangs-Randall | Texas | Sun Belt |
| Dulins Dodgers | Tennessee | Deep South |
| East Coast Grays North | New York | New England |
| East Coast Grays South | Florida | Sunshine |
| East Cobb Astros 17's | Georgia | Deep South |
| East Tennessee Padres | Tennessee | Deep South |
| EDH Vipers | California | West Coast |
| Farrah Builders | New Jersey | New England |
| Florida Hardballers | Florida | Sunshine |
| Frozen Ropes Texas | Texas | Sun Belt |
| Hard 90 Baseball | California | West Coast |
| Huntington Hounds | West Virginia | Ohio Valley |
| Kentucky Baseball Club | Kentucky | Ohio Valley |
| Longshots Baseball | Illinois | Great Plains |
| Mac-N-Seitz Indians | Missouri | Great Plains |
| Marucci Royals | Louisiana | Sun Belt |
| Mid-Atlantic Red Sox | Maryland | Atlantic Coast |
| Midland Indians | Ohio | Ohio Valley |
| Mizuno Titans | New York | New England |
| New Jersey Marlins | New Jersey | New England |
| New Jersey Twins | New Jersey | New England |
| Northeast Rays | Massachusetts | New England |
| NVTBL Stars | Virginia | Atlantic Coast |
| On Deck O's | North Carolina | Atlantic Coast |
| Orioles Scout Team | Maryland | Atlantic Coast |
| Palmetto Sand Gnats | South Carolina | Atlantic Coast |
| Philadelphia Senators | Pennsylvania | Ohio Valley |
| Richmond Braves National | Virginia | Atlantic Coast |
| San Gabriel Valley Arsenal | California | West Coast |
| Schaumburg Seminoles | Illinois | Great Plains |
| Seattle Select | Washington | Pacific Northwest |
| So Cal Angels | California | West Coast |
| South Bergen Mets | New York | New England |
| South Charlotte Panthers | North Carolina | Atlantic Coast |
| South Florida Elite | Florida | Sunshine |
| South Oakland A's | Michigan | Ohio Valley |
| South Troy Dodgers | New York | New England |
| St Louis Pirates | Missouri | Great Plains |
| Syracuse Sports Zone Braves | New York | New England |
| Tampa Bay Warriors | Florida | Sunshine |
| Team Elite | Georgia | Deep South |
| Team Georgia | Georgia | Deep South |
| Tri-State Arsenal | New Jersey | New England |
| Upstate Mavericks | South Carolina | Atlantic Coast |
| Virginia Barnstormers | Virginia | Atlantic Coast |
| West Florida Shockers | Florida | Sunshine |
| World of Baseball | Florida | Sunshine |