| 1 | South Florida Elite Black | Florida | Sunshine |
| 2 | Houston Banditos Black | Texas | Sun Belt |
| 3 | Orlando Scorpions Underclass Purple | Florida | Sunshine |
| 4 | Canes White | Virginia | Atlantic Coast |
| 5 | East Cobb Titans | Georgia | Deep South |
| 6 | Dulins Dodgers | Tennessee | Deep South |
| 7 | Palm Beach County PAL | Florida | Sunshine |
| 8 | Marucci Ark La Tex | Louisiana | Sun Belt |
| 9 | NorCal Baseball | California | West Coast |
| 10 | Team Elite White | Georgia | Deep South |
| 11 | Richmond Braves National | Virginia | Atlantic Coast |
| 12 | SC Shockwave | South Carolina | Atlantic Coast |
| 13 | SF Prospects | Florida | Sunshine |
| 14 | Chet Lemon's Juice | Florida | Sunshine |
| 15 | Indiana Prospects | Indiana | Ohio Valley |
| 16 | Midland Braves | Ohio | Ohio Valley |
| 17 | Excel Blue Sox | Alabama | Deep South |
| 18 | CBC Riverhawks | Florida | Sunshine |
| 19 | Marucci Elite | Louisiana | Sun Belt |
| 20 | So Cal NTT | California | West Coast |
| 21 | Kentucky Baseball Club | Kentucky | Ohio Valley |
| 22 | D-Bat Mustangs-Sharp | Texas | Sun Belt |
| 23 | South Charlotte Panthers | North Carolina | Atlantic Coast |
| 24 | 6-4-3 DP Cougars | Georgia | Deep South |
| 25 | Houston Heat | Texas | Sun Belt |
